Coding

Part:BBa_K4345013

Designed by: Michelle Patricia   Group: iGEM22_KU_Leuven   (2022-09-30)


PfdeAR

PfdeAR is a native bidirectional promotor of LysR-type PfdeAR-FdeR pair from Herbaspirillum seropedicae. PfdeAR is a part of PfdeAR-FdeR biosensor circuit from the naringenin-responsive transcriptional biosensor develop by De Paepe et al. (2018).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7

Usage and Biology

This part is part of the biosensor circuit shown below.


PSynSense2X De Paepe et al 2018.png

Image obtained from De Paepe et. al. (2018)

References

De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419

[edit]
Categories
//promoter
Parameters
control